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Updated: Aug 01, 2026

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ASCP ASCP-MLT Exam Overview:

Certification Vendor:American Society for Clinical Pathology Board of Certification (ASCP BOC)
Exam Name:Medical Laboratory Technician
Exam Number:MLT(ASCP)
Real Exam Qty:Approximately 100
Exam Duration:150 minutes
Related Certifications:MLS(ASCP) Medical Laboratory Scientist
PBT(ASCP) Phlebotomy Technician
Exam Price:Application fee (USD 235–250, varies by region and international discounts)
Certificate Validity Period:Certification maintenance required (completion of ongoing continuing education every 3 years)
Exam Format:Computer Adaptive Testing (CAT), Multiple-choice questions (one best answer)
Passing Score:Scaled score of ≥ 400 (on 100–999 scale, CAT scoring)
Available Languages:English
Sample Questions:ASCP ASCP-MLT Sample Questions
Exam Way:Computer-based testing delivered at Pearson VUE test centres
Pre Condition:Completion of accredited MLT program or equivalent education/training OR relevant clinical experience following ASCP BOC eligibility routes
Official Syllabus URL:https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT

ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Laboratory Operations5–10%- Quality assessment and safety
- Instrumentation and laboratory mathematics
Microbiology15–20%- Virology and parasitology testing
- Bacteriology and mycology procedures
Blood Banking15–20%- Transfusion practice and testing
- Blood group systems and immunology
- Blood products and components
Urinalysis and Other Body Fluids5–10%- Physical and chemical testing
- Microscopic analysis and disease correlation
Immunology5–10%- Principles of immunology
- Serologic procedures and immune diseases
Chemistry20–25%- Carbohydrates, lipids, and enzymes
- Electrolytes, acid-base, and toxicology
Hematology20–25%- Laboratory testing and hemostasis
- Physiology and disease states

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. If Kappa or Lambda is predominant and CD5 is co-expressed with CD19 (CD19/CD5 dual positive lymphocyte population), and CD23 is expressed, chronic lymphocytic leukemia is a probable diagnosis. CD19 is normally found on normal B cell populations and CD5 is normally found on mature T cell populations.
However, CD5 is present on B cells in B-chronic lymphocytic leukemia or Mantle Cell Lymphoma, both abnormal B cell malignant processes.
CD23 expression is not consistent with Mantel cells as shown on the decision tree on the right.
A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following?

A) Mantle cell lymphoma
B) A normal B cell
C) Chronic lymphocytic leukemia
D) A mature T cell


2. A dilution commonly used for a routine sperm count is a 1:20.
A dilution commonly used for a routine sperm count is:

A) 1:2
B) 1:400
C) 1:200
D) 1:20


3. Shigella and Salmonella are not found as normal flora in the gastrointestinal tract.
Which of the following are not considered normal flora of the gastrointestinal tract:

A) Lactobacillus
B) Shigella
C) Peptostreptococcus
D) Clostridium


4. Provide the equivalent measurement for one pint.

A) 0.1 liter
B) 0.474 liters
C) 4.2 liters
D) 0.25 liters


5. PCT usually rises within 3-6 hours of infection. CRP also increases rapidly following infection, but is not as specific for infection as PCT. A rise in CRP could also occur with SIRS. Lactic acid is usually used to detect and monitor impaired circulation and tissue oxygenation in critically ill patients.
Chemistry
Of the three laboratory tests that are listed, which has proven to be most effective for early differentiation of systemic inflammatory response syndrome (SIRS) from sepsis due to its increase following infection and higher specificity?

A) Lactic acid
B) Procalcitonin (PCT)
C) C-reactive protein (CRP)


Solutions:

Question # 1
Answer: C
Question # 2
Answer: D
Question # 3
Answer: B
Question # 4
Answer: B
Question # 5
Answer: B

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